I've been using E85 on customer's cars for a while now. We run up to Turkey Lake Service Plaza in Orlando. Fill 54 gallon barrels. Some times two or three at a time. Lasts us a bit. With fuel only tuning I usually see a minimum of 30whp across the board (turbo applications). With fuel and timing tuning I usually see a minimum of 50whp across the board. Spoolup is roughly 300rshared_pm - 600rshared_pm sooner as well